A single living bacterium's refractive index measurement by using optofluidic immersion refractometry

Y. Liu, L. K. Chin, W. Ser, T. C. Ayi, W. M. Ho, P. H. Yap, Y. Leprince-Wang, T. Bourouina

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

3 Citations (Scopus)

Abstract

This paper presents a biophysical method to characterize single bacterium in water by using an on-chip immersion refractometer. The working principle is based on immersion refractometry, whereby the refractive index of a single bacterium is measured using the difference between the various refractive indices. The preliminary results show that E. coli is larger in term of width and has a lower refractive index value as compared to bacillus subtilis. The database for protozoa will be expanded by measuring different kinds of bacteria using the on-chip immersion refractometer.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages263-265
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 Oct 201331 Oct 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords

  • Bacillus subtilis
  • Escherichia coli
  • Optofluidic immersion refractometry
  • Refractive index

ASJC Scopus subject areas

  • Bioengineering

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