A signal-on fluorescence biosensor for detection of adenosine triphosphate based on click chemistry

Ning Sun, Qi Guo, Jingwei Shao, Bin Qiu, Zhenyu Lin, Kwok Yin Wong, Guonan Chen

Research output: Journal article publicationJournal articleAcademic researchpeer-review

11 Citations (Scopus)

Abstract

A novel signal-on fluorescence biosensor for detection of adenosine triphosphate (ATP) based on the target induced recombination of split aptamer fragments and click chemistry is proposed. The CuS NPs modified on the streptavidin magnesphere paramagnetic particles (PMPs) can be destroyed by acid to form Cu(ii). In the presence of sodium ascorbate, Cu(ii) can be reduced to Cu(i) which in turn catalyzes the reaction between a weak-fluorescent 3-azido-7-hydroxycoumarin and propargyl alcohol to form strongly fluorescent 1,2,3-triazole compounds. The proposed sensor has been applied to the detection of ATP in human serum samples with satisfactory results.
Original languageEnglish
Pages (from-to)3370-3374
Number of pages5
JournalAnalytical Methods
Volume6
Issue number10
DOIs
Publication statusPublished - 21 May 2014

ASJC Scopus subject areas

  • Analytical Chemistry
  • General Chemical Engineering
  • General Engineering

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