A signal-on fluorescence biosensor for detection of adenosine triphosphate based on click chemistry

Ning Sun; Qi Guo; Jingwei Shao; Bin Qiu; Zhenyu Lin; Kwok Yin Wong; Guonan Chen

doi:10.1039/c4ay00220b

A signal-on fluorescence biosensor for detection of adenosine triphosphate based on click chemistry

Ning Sun, Qi Guo, Jingwei Shao, Bin Qiu, Zhenyu Lin, Kwok Yin Wong, Guonan Chen

Research output: Journal article publication › Journal article › Academic research › peer-review

12 Citations (Scopus)

Abstract

A novel signal-on fluorescence biosensor for detection of adenosine triphosphate (ATP) based on the target induced recombination of split aptamer fragments and click chemistry is proposed. The CuS NPs modified on the streptavidin magnesphere paramagnetic particles (PMPs) can be destroyed by acid to form Cu(ii). In the presence of sodium ascorbate, Cu(ii) can be reduced to Cu(i) which in turn catalyzes the reaction between a weak-fluorescent 3-azido-7-hydroxycoumarin and propargyl alcohol to form strongly fluorescent 1,2,3-triazole compounds. The proposed sensor has been applied to the detection of ATP in human serum samples with satisfactory results.

Original language	English
Pages (from-to)	3370-3374
Number of pages	5
Journal	Analytical Methods
Volume	6
Issue number	10
DOIs	https://doi.org/10.1039/c4ay00220b
Publication status	Published - 21 May 2014

ASJC Scopus subject areas

Analytical Chemistry
General Chemical Engineering
General Engineering

More information

10.1039/c4ay00220b

Cite this

@article{e782a523fd6c4101a1af28c1a7eda2f7,

title = "A signal-on fluorescence biosensor for detection of adenosine triphosphate based on click chemistry",

abstract = "A novel signal-on fluorescence biosensor for detection of adenosine triphosphate (ATP) based on the target induced recombination of split aptamer fragments and click chemistry is proposed. The CuS NPs modified on the streptavidin magnesphere paramagnetic particles (PMPs) can be destroyed by acid to form Cu(ii). In the presence of sodium ascorbate, Cu(ii) can be reduced to Cu(i) which in turn catalyzes the reaction between a weak-fluorescent 3-azido-7-hydroxycoumarin and propargyl alcohol to form strongly fluorescent 1,2,3-triazole compounds. The proposed sensor has been applied to the detection of ATP in human serum samples with satisfactory results.",

author = "Ning Sun and Qi Guo and Jingwei Shao and Bin Qiu and Zhenyu Lin and Wong, {Kwok Yin} and Guonan Chen",

year = "2014",

month = may,

day = "21",

doi = "10.1039/c4ay00220b",

language = "English",

volume = "6",

pages = "3370--3374",

journal = "Analytical Methods",

issn = "1759-9660",

publisher = "Royal Society of Chemistry",

number = "10",

}

TY - JOUR

T1 - A signal-on fluorescence biosensor for detection of adenosine triphosphate based on click chemistry

AU - Sun, Ning

AU - Guo, Qi

AU - Shao, Jingwei

AU - Qiu, Bin

AU - Lin, Zhenyu

AU - Wong, Kwok Yin

AU - Chen, Guonan

PY - 2014/5/21

Y1 - 2014/5/21

N2 - A novel signal-on fluorescence biosensor for detection of adenosine triphosphate (ATP) based on the target induced recombination of split aptamer fragments and click chemistry is proposed. The CuS NPs modified on the streptavidin magnesphere paramagnetic particles (PMPs) can be destroyed by acid to form Cu(ii). In the presence of sodium ascorbate, Cu(ii) can be reduced to Cu(i) which in turn catalyzes the reaction between a weak-fluorescent 3-azido-7-hydroxycoumarin and propargyl alcohol to form strongly fluorescent 1,2,3-triazole compounds. The proposed sensor has been applied to the detection of ATP in human serum samples with satisfactory results.

AB - A novel signal-on fluorescence biosensor for detection of adenosine triphosphate (ATP) based on the target induced recombination of split aptamer fragments and click chemistry is proposed. The CuS NPs modified on the streptavidin magnesphere paramagnetic particles (PMPs) can be destroyed by acid to form Cu(ii). In the presence of sodium ascorbate, Cu(ii) can be reduced to Cu(i) which in turn catalyzes the reaction between a weak-fluorescent 3-azido-7-hydroxycoumarin and propargyl alcohol to form strongly fluorescent 1,2,3-triazole compounds. The proposed sensor has been applied to the detection of ATP in human serum samples with satisfactory results.

UR - http://www.scopus.com/inward/record.url?scp=84899511079&partnerID=8YFLogxK

U2 - 10.1039/c4ay00220b

DO - 10.1039/c4ay00220b

M3 - Journal article

SN - 1759-9660

VL - 6

SP - 3370

EP - 3374

JO - Analytical Methods

JF - Analytical Methods

IS - 10

ER -

A signal-on fluorescence biosensor for detection of adenosine triphosphate based on click chemistry

Abstract

ASJC Scopus subject areas

More information

Other files and links

Fingerprint

Cite this