@article{8a3c9f898ce148aeb53aac6645729e50,
title = "A sensitive and specific competitive enzyme-linked immunosorbent assay for serodiagnosis of covid-19 in animals",
abstract = "In addition to human cases, cases of COVID-19 in captive animals and pets are increas-ingly reported. This raises the concern for two-way COVID-19 transmission between humans and animals. Here, we developed a SARS-CoV-2 nucleocapsid protein-based competitive enzyme-linked immunosorbent assay (cELISA) for serodiagnosis of COVID-19 which can theoretically be used in virtually all kinds of animals. We used 187 serum samples from patients with/without COVID-19, laboratory animals immunized with inactive SARS-CoV-2 virions, COVID-19-negative animals, and animals seropositive to other betacoronaviruses. A cut-off percent inhibition value of 22.345\% was determined and the analytical sensitivity and specificity were found to be 1:64–1:256 and 93.9\%, respectively. Evaluation on its diagnostic performance using 155 serum samples from COVID-19-negative animals and COVID-19 human patients showed a diagnostic sensitivity and specificity of 80.8\% and 100\%, respectively. The cELISA can be incorporated into routine blood testing of farmed/captive animals for COVID-19 surveillance.",
keywords = "Antibody, Competitive ELISA, COVID-19, Nucleocapsid protein, SARS-CoV-2",
author = "Lau, \{Susanna K.P.\} and Zirong He and Tsang, \{Chi Ching\} and Chan, \{Tony T.Y.\} and Luk, \{Hayes K.H.\} and Elaine Chan and Li, \{Kenneth S.M.\} and Joshua Fung and Chow, \{Franklin W.N.\} and Tam, \{Anthony R.\} and Chung, \{Tom W.H.\} and Wong, \{Sally C.Y.\} and Que, \{Tak Lun\} and Fung, \{Kitty S.C.\} and Lung, \{David C.\} and Wu, \{Alan K.L.\} and Hung, \{Ivan F.N.\} and Teng, \{Jade L.L.\} and Ulrich Wernery and Hui, \{Suk Wai\} and Paolo Martelli and Woo, \{Patrick C.Y.\}",
note = "Funding Information: Funding: This work was supported by the Innovation and Technology Fund Public Sector Trial Scheme—Special Call for Projects for the Prevention and Control of Coronavirus Disease 2019 (COVID-19) in Hong Kong (PSTS-COVID-19) (SST/117/20GP) of the Innovation and Technology Commission, the Government of the Hong Kong Special Administrative Region. Any opinions, findings, conclusions, or recommendations expressed in this material/event (or by members of the project team) do not reflect the views of the Government of the Hong Kong Special Administrative Region, the Innovation and Technology Commission or the Innovation and Technology Fund Research Projects Assessment Panel. Funding Information: This work was supported by the Innovation and Technology Fund Public Sector Trial Scheme?Special Call for Projects for the Prevention and Control of Coronavirus Disease 2019 (COVID-19) in Hong Kong (PSTS-COVID-19) (SST/117/20GP) of the Innovation and Technology Commission, the Government of the Hong Kong Special Administrative Region. Any opinions, findings, conclusions, or recommendations expressed in this material/event (or by members of the project team) do not reflect the views of the Government of the Hong Kong Special Administrative Region, the Innovation and Technology Commission or the Innovation and Technology Fund Research Projects Assessment Panel. We thank the staff from the Veterinary Public Health Section, Food and Environmental Hygiene Department of the Hong Kong Government for their help in collecting the porcine serum samples. We are also grateful to Kwok-Yung Yuen for his help in obtaining some of the serum samples. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = may,
doi = "10.3390/microorganisms9051019",
language = "English",
volume = "9",
journal = "Microorganisms",
issn = "2076-2607",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "5",
}