TY - JOUR
T1 - A rabbit osteochondral defect (OCD) model for evaluation of tissue engineered implants on their biosafety and efficacy in osteochondral repair
AU - Zhou, Liangbin
AU - Ho, Ki Wai Kevin
AU - Zheng, Lizhen
AU - Xu, Jiankun
AU - Chen, Ziyi
AU - Ye, Xiangdong
AU - Zou, Li
AU - Li, Ye
AU - Chang, Liang
AU - Shao, Hongwei
AU - Li, Xisheng
AU - Long, Jing
AU - Nie, Yangyi
AU - Stoddart, Martin J.
AU - Lai, Yuxiao
AU - Qin, Ling
N1 - Publisher Copyright:
Copyright © 2024 Zhou, Ho, Zheng, Xu, Chen, Ye, Zou, Li, Chang, Shao, Li, Long, Nie, Stoddart, Lai and Qin.
PY - 2024/5/3
Y1 - 2024/5/3
N2 - Osteochondral defect (OCD) is a common but challenging condition in orthopaedics that imposes huge socioeconomic burdens in our aging society. It is imperative to accelerate the R&D of regenerative scaffolds using osteochondral tissue engineering concepts. Yet, all innovative implant-based treatments require animal testing models to verify their feasibility, biosafety, and efficacy before proceeding to human trials. Rabbit models offer a more clinically relevant platform for studying OCD repair than smaller rodents, while being more cost-effective than large animal models. The core-decompression drilling technique to produce full-thickness distal medial femoral condyle defects in rabbits can mimic one of the trauma-relevant OCD models. This model is commonly used to evaluate the implant’s biosafety and efficacy of osteochondral dual-lineage regeneration. In this article, we initially indicate the methodology and describe a minimally-invasive surgical protocol in a step-wise manner to generate a standard and reproducible rabbit OCD for scaffold implantation. Besides, we provide a detailed procedure for sample collection, processing, and evaluation by a series of subsequent standardized biochemical, radiological, biomechanical, and histological assessments. In conclusion, the well-established, easy-handling, reproducible, and reliable rabbit OCD model will play a pivotal role in translational research of osteochondral tissue engineering.
AB - Osteochondral defect (OCD) is a common but challenging condition in orthopaedics that imposes huge socioeconomic burdens in our aging society. It is imperative to accelerate the R&D of regenerative scaffolds using osteochondral tissue engineering concepts. Yet, all innovative implant-based treatments require animal testing models to verify their feasibility, biosafety, and efficacy before proceeding to human trials. Rabbit models offer a more clinically relevant platform for studying OCD repair than smaller rodents, while being more cost-effective than large animal models. The core-decompression drilling technique to produce full-thickness distal medial femoral condyle defects in rabbits can mimic one of the trauma-relevant OCD models. This model is commonly used to evaluate the implant’s biosafety and efficacy of osteochondral dual-lineage regeneration. In this article, we initially indicate the methodology and describe a minimally-invasive surgical protocol in a step-wise manner to generate a standard and reproducible rabbit OCD for scaffold implantation. Besides, we provide a detailed procedure for sample collection, processing, and evaluation by a series of subsequent standardized biochemical, radiological, biomechanical, and histological assessments. In conclusion, the well-established, easy-handling, reproducible, and reliable rabbit OCD model will play a pivotal role in translational research of osteochondral tissue engineering.
KW - implant
KW - osteochondral defect
KW - rabbit model
KW - tissue engineering
KW - translational research
UR - http://www.scopus.com/inward/record.url?scp=85193679607&partnerID=8YFLogxK
U2 - 10.3389/fbioe.2024.1352023
DO - 10.3389/fbioe.2024.1352023
M3 - Journal article
AN - SCOPUS:85193679607
SN - 2296-4185
VL - 12
JO - Frontiers in Bioengineering and Biotechnology
JF - Frontiers in Bioengineering and Biotechnology
M1 - 1352023
ER -