A Probe Based on a Soft Salt Complex for Ratiometric and Lifetime Imaging of Variations in Intracellular Oxygen Content

Yun Ma; Yafang Dong; Liang Zou; Liang Shen; Suyi Liu; Shujuan Liu; Wei Huang; Qiang Zhao; Wai Yeung Wong

doi:10.1002/ejic.201800250

A Probe Based on a Soft Salt Complex for Ratiometric and Lifetime Imaging of Variations in Intracellular Oxygen Content

Yun Ma, Yafang Dong, Liang Zou, Liang Shen, Suyi Liu, Shujuan Liu, Wei Huang, Qiang Zhao, Wai Yeung Wong

Research output: Journal article publication › Journal article › Academic research › peer-review

11 Citations (Scopus)

Abstract

In this study, a soft salt complex based probe has been exploited for measuring oxygen content changes in living cells. The zinc(II) complex [Zn(tpy)₂]²⁺(CH₃COO^–)₂ {tpy = 3-[(2,2′:6′,2′′-terpyridin)-4′-yl]-9-hexyl-9H-carbazole} was chosen as the cationic component, and the iridium(III) complex [Ir(tpq)₂CN₂]^–Bu₄N⁺ [tpq = 2-(thiophen-2-yl)quinoline] was selected as the anionic component. The soft salt complex based probe was formed through electrostatic interaction between two oppositely charged complexes. This probe exhibits ratiometric photoluminescence response to different oxygen concentrations with two separated emission bands. Furthermore, the application of this probe in ratiometric and lifetime imaging of oxygen content changes in living cells has been demonstrated.

Original language	English
Pages (from-to)	2345-2349
Number of pages	5
Journal	European Journal of Inorganic Chemistry
Volume	2018
Issue number	20
DOIs	https://doi.org/10.1002/ejic.201800250
Publication status	Published - 7 Jun 2018

Keywords

Fluorescent probes
Imaging agents
Oxygen
Ratiometric imaging
Soft salt complexes

ASJC Scopus subject areas

Inorganic Chemistry

More information

10.1002/ejic.201800250

Cite this

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title = "A Probe Based on a Soft Salt Complex for Ratiometric and Lifetime Imaging of Variations in Intracellular Oxygen Content",

abstract = "In this study, a soft salt complex based probe has been exploited for measuring oxygen content changes in living cells. The zinc(II) complex [Zn(tpy)2]2+(CH3COO–)2 {tpy = 3-[(2,2′:6′,2′′-terpyridin)-4′-yl]-9-hexyl-9H-carbazole} was chosen as the cationic component, and the iridium(III) complex [Ir(tpq)2CN2]–Bu4N+ [tpq = 2-(thiophen-2-yl)quinoline] was selected as the anionic component. The soft salt complex based probe was formed through electrostatic interaction between two oppositely charged complexes. This probe exhibits ratiometric photoluminescence response to different oxygen concentrations with two separated emission bands. Furthermore, the application of this probe in ratiometric and lifetime imaging of oxygen content changes in living cells has been demonstrated.",

keywords = "Fluorescent probes, Imaging agents, Oxygen, Ratiometric imaging, Soft salt complexes",

author = "Yun Ma and Yafang Dong and Liang Zou and Liang Shen and Suyi Liu and Shujuan Liu and Wei Huang and Qiang Zhao and Wong, {Wai Yeung}",

year = "2018",

month = jun,

day = "7",

doi = "10.1002/ejic.201800250",

language = "English",

volume = "2018",

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journal = "European Journal of Inorganic Chemistry",

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T1 - A Probe Based on a Soft Salt Complex for Ratiometric and Lifetime Imaging of Variations in Intracellular Oxygen Content

AU - Ma, Yun

AU - Dong, Yafang

AU - Zou, Liang

AU - Shen, Liang

AU - Liu, Suyi

AU - Liu, Shujuan

AU - Huang, Wei

AU - Zhao, Qiang

AU - Wong, Wai Yeung

PY - 2018/6/7

Y1 - 2018/6/7

N2 - In this study, a soft salt complex based probe has been exploited for measuring oxygen content changes in living cells. The zinc(II) complex [Zn(tpy)2]2+(CH3COO–)2 {tpy = 3-[(2,2′:6′,2′′-terpyridin)-4′-yl]-9-hexyl-9H-carbazole} was chosen as the cationic component, and the iridium(III) complex [Ir(tpq)2CN2]–Bu4N+ [tpq = 2-(thiophen-2-yl)quinoline] was selected as the anionic component. The soft salt complex based probe was formed through electrostatic interaction between two oppositely charged complexes. This probe exhibits ratiometric photoluminescence response to different oxygen concentrations with two separated emission bands. Furthermore, the application of this probe in ratiometric and lifetime imaging of oxygen content changes in living cells has been demonstrated.

AB - In this study, a soft salt complex based probe has been exploited for measuring oxygen content changes in living cells. The zinc(II) complex [Zn(tpy)2]2+(CH3COO–)2 {tpy = 3-[(2,2′:6′,2′′-terpyridin)-4′-yl]-9-hexyl-9H-carbazole} was chosen as the cationic component, and the iridium(III) complex [Ir(tpq)2CN2]–Bu4N+ [tpq = 2-(thiophen-2-yl)quinoline] was selected as the anionic component. The soft salt complex based probe was formed through electrostatic interaction between two oppositely charged complexes. This probe exhibits ratiometric photoluminescence response to different oxygen concentrations with two separated emission bands. Furthermore, the application of this probe in ratiometric and lifetime imaging of oxygen content changes in living cells has been demonstrated.

KW - Fluorescent probes

KW - Imaging agents

KW - Oxygen

KW - Ratiometric imaging

KW - Soft salt complexes

UR - http://www.scopus.com/inward/record.url?scp=85048048755&partnerID=8YFLogxK

U2 - 10.1002/ejic.201800250

DO - 10.1002/ejic.201800250

M3 - Journal article

AN - SCOPUS:85048048755

SN - 1434-1948

VL - 2018

SP - 2345

EP - 2349

JO - European Journal of Inorganic Chemistry

JF - European Journal of Inorganic Chemistry

IS - 20

ER -

A Probe Based on a Soft Salt Complex for Ratiometric and Lifetime Imaging of Variations in Intracellular Oxygen Content

Abstract

Keywords

ASJC Scopus subject areas

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