A photoelectrochemical biosensor for rapid and ultrasensitive norovirus detection

Jiubiao Guo; Dan Liu; Zhiqiang Yang; Wenchuan Weng; Edward Wai Chi Chan; Zhenling Zeng; Kwok Yin Wong; Peng Lin; Sheng Chen

doi:10.1016/j.bioelechem.2020.107591

A photoelectrochemical biosensor for rapid and ultrasensitive norovirus detection

Jiubiao Guo, Dan Liu, Zhiqiang Yang, Wenchuan Weng, Edward Wai Chi Chan, Zhenling Zeng, Kwok Yin Wong, Peng Lin, Sheng Chen

Research output: Journal article publication › Journal article › Academic research › peer-review

25 Citations (Scopus)

Abstract

The highly contagious norovirus (NoV) is the most common causative agent of acute gastroenteritis, resulting in >200,000 deaths worldwide annually. A rapid and sensitive detection method is a prerequisite for effective prevention and timely identification of NoV contamination. In the present study, we developed a photoelectrochemical (PEC) biosensor coupled with a novel custom-made monoclonal antibody (mAb) for specific and sensitive NoV detection. Our system could detect levels of recombinant NoV capsid protein VP1 as low as 2 × 10⁻¹⁰ g mL⁻¹ (4.9 pM) within 30 min in a concentration-dependent manner. More importantly, the biosensor was versatile in detecting virus isolated from real samples that were as low as 46 copies μL⁻¹. These findings indicate that this system has the potential to serve as a convenient point-of-care system for diagnosing NoV infection and detecting NoV-contaminated food samples.

Original language	English
Article number	107591
Journal	Bioelectrochemistry
Volume	136
DOIs	https://doi.org/10.1016/j.bioelechem.2020.107591
Publication status	Published - Dec 2020
Externally published	Yes

Keywords

Monoclonal antibody
Norovirus
Photoelectrochemical biosensor
QD
VP1

ASJC Scopus subject areas

Biophysics
Physical and Theoretical Chemistry
Electrochemistry

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10.1016/j.bioelechem.2020.107591

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title = "A photoelectrochemical biosensor for rapid and ultrasensitive norovirus detection",

abstract = "The highly contagious norovirus (NoV) is the most common causative agent of acute gastroenteritis, resulting in >200,000 deaths worldwide annually. A rapid and sensitive detection method is a prerequisite for effective prevention and timely identification of NoV contamination. In the present study, we developed a photoelectrochemical (PEC) biosensor coupled with a novel custom-made monoclonal antibody (mAb) for specific and sensitive NoV detection. Our system could detect levels of recombinant NoV capsid protein VP1 as low as 2 × 10−10 g mL−1 (4.9 pM) within 30 min in a concentration-dependent manner. More importantly, the biosensor was versatile in detecting virus isolated from real samples that were as low as 46 copies μL−1. These findings indicate that this system has the potential to serve as a convenient point-of-care system for diagnosing NoV infection and detecting NoV-contaminated food samples.",

keywords = "Monoclonal antibody, Norovirus, Photoelectrochemical biosensor, QD, VP1",

author = "Jiubiao Guo and Dan Liu and Zhiqiang Yang and Wenchuan Weng and Chan, {Edward Wai Chi} and Zhenling Zeng and Wong, {Kwok Yin} and Peng Lin and Sheng Chen",

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doi = "10.1016/j.bioelechem.2020.107591",

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AU - Guo, Jiubiao

AU - Liu, Dan

AU - Yang, Zhiqiang

AU - Weng, Wenchuan

AU - Chan, Edward Wai Chi

AU - Zeng, Zhenling

AU - Wong, Kwok Yin

AU - Lin, Peng

AU - Chen, Sheng

PY - 2020/12

Y1 - 2020/12

N2 - The highly contagious norovirus (NoV) is the most common causative agent of acute gastroenteritis, resulting in >200,000 deaths worldwide annually. A rapid and sensitive detection method is a prerequisite for effective prevention and timely identification of NoV contamination. In the present study, we developed a photoelectrochemical (PEC) biosensor coupled with a novel custom-made monoclonal antibody (mAb) for specific and sensitive NoV detection. Our system could detect levels of recombinant NoV capsid protein VP1 as low as 2 × 10−10 g mL−1 (4.9 pM) within 30 min in a concentration-dependent manner. More importantly, the biosensor was versatile in detecting virus isolated from real samples that were as low as 46 copies μL−1. These findings indicate that this system has the potential to serve as a convenient point-of-care system for diagnosing NoV infection and detecting NoV-contaminated food samples.

AB - The highly contagious norovirus (NoV) is the most common causative agent of acute gastroenteritis, resulting in >200,000 deaths worldwide annually. A rapid and sensitive detection method is a prerequisite for effective prevention and timely identification of NoV contamination. In the present study, we developed a photoelectrochemical (PEC) biosensor coupled with a novel custom-made monoclonal antibody (mAb) for specific and sensitive NoV detection. Our system could detect levels of recombinant NoV capsid protein VP1 as low as 2 × 10−10 g mL−1 (4.9 pM) within 30 min in a concentration-dependent manner. More importantly, the biosensor was versatile in detecting virus isolated from real samples that were as low as 46 copies μL−1. These findings indicate that this system has the potential to serve as a convenient point-of-care system for diagnosing NoV infection and detecting NoV-contaminated food samples.

KW - Monoclonal antibody

KW - Norovirus

KW - Photoelectrochemical biosensor

KW - QD

KW - VP1

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DO - 10.1016/j.bioelechem.2020.107591

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SN - 1567-5394

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JO - Bioelectrochemistry

JF - Bioelectrochemistry

M1 - 107591

ER -

A photoelectrochemical biosensor for rapid and ultrasensitive norovirus detection

Abstract

Keywords

ASJC Scopus subject areas

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