Abstract
This paper presents a new PDMS planar patch-clamp array chip integrated with microfluidic units and a poly (ethylene glycol)/SU-8 mixture based cell-patch interface for whole cell recordings. In our experiments, the PEG/SU-8 mixture with the weight ratio of 1:1 has been proven to have improved properties to fabricate perfect apertures compared with PDMS for cell-patch interface, such as large Young's modulus and good hydrophilic surface properties. The integrated chip was tested to trap 20μm polystyrene microsphere onto 2μm aperture by a negative pressure, and the variation of impedance spectra in trapping process demonstrated that the excellent sealing resistance has been achieved. Our present results indicate the potential of a planar patch-clamp array chip system with PEG/SU-8 base cell-patch interface for high-throughput whole cell recording.
Original language | English |
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Article number | 4805402 |
Pages (from-to) | 395-398 |
Number of pages | 4 |
Journal | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) |
DOIs | |
Publication status | Published - 1 Jun 2009 |
Event | 22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 - Sorrento, Italy Duration: 25 Jan 2009 → 29 Jan 2009 |
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Mechanical Engineering
- Condensed Matter Physics
- Electronic, Optical and Magnetic Materials