A novel PCR-based approach for accurate identification of Vibrio parahaemolyticus

Ruichao Li, Jia-chi Chiou, Edward Wai Chi Chan, Sheng Chen

Research output: Journal article publicationJournal articleAcademic researchpeer-review

17 Citations (Scopus)

Abstract

A PCR-based assay was developed for more accurate identification of Vibrio parahaemolyticus through targeting the blaCARB-17 like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. Homologous analysis showed that blaCARB-17 like genes were more conservative than the tlh, toxR and atpA genes, the genetic markers commonly used as detection targets in identification of V. parahaemolyticus. Our data showed that this blaCARB-17-specific PCR-based detection approach consistently achieved 100% specificity, whereas PCR targeting the tlh and atpA genes occasionally produced false positive results. Furthermore, a positive result of this test is consistently associated with an intrinsic ampicillin resistance phenotype of the test organism, presumably conferred by the products of blaCARB-17 like genes. We envision that combined analysis of the unique genetic and phenotypic characteristics conferred by blaCARB-17 shall further enhance the detection specificity of this novel yet easy-to-use detection approach to a level superior to the conventional methods used in V. parahaemolyticus detection and identification.
Original languageEnglish
Article number00044
JournalFrontiers in Microbiology
Volume7
Issue numberJAN
DOIs
Publication statusPublished - 1 Jan 2016

Keywords

  • Bla CARB-17
  • Molecular detection
  • PCR
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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