A novel method to prepare chitosan powder and its application in cellulase immobilization

Xuepu Mao, Gangjun Guo, Jinfeng Huang, Zhiyun Du, Zhishu Huang, Lin Ma, Pei Li, Lianquan Gu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

49 Citations (Scopus)

Abstract

In this study, chitosan microspheres and sponges with uniform spherical and porous morphologies were prepared by coiling the stretched chains of chitosan with addition of salt and choosing different kinds of organic solvents as evaporation solvents. Cellulase was immobilized to the support by a covalent method. The enzyme exhibited a considerable affinity to the support, and the protein loading of 145.5 mg g-1support was fairly high. The immobilized cellulase had a higher Kmthan free cellulase and had better stability with respect to pH, thermal stability, reuses and storage stability than free cellulase.
Original languageEnglish
Pages (from-to)189-195
Number of pages7
JournalJournal of Chemical Technology and Biotechnology
Volume81
Issue number2
DOIs
Publication statusPublished - 1 Feb 2006

Keywords

  • Ammonium sulfate
  • Cellulase
  • Chitosan
  • Immobilized
  • Microsphere
  • Sponge

ASJC Scopus subject areas

  • Biotechnology
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Inorganic Chemistry

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