Abstract
In this study, chitosan microspheres and sponges with uniform spherical and porous morphologies were prepared by coiling the stretched chains of chitosan with addition of salt and choosing different kinds of organic solvents as evaporation solvents. Cellulase was immobilized to the support by a covalent method. The enzyme exhibited a considerable affinity to the support, and the protein loading of 145.5 mg g-1support was fairly high. The immobilized cellulase had a higher Kmthan free cellulase and had better stability with respect to pH, thermal stability, reuses and storage stability than free cellulase.
Original language | English |
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Pages (from-to) | 189-195 |
Number of pages | 7 |
Journal | Journal of Chemical Technology and Biotechnology |
Volume | 81 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Feb 2006 |
Keywords
- Ammonium sulfate
- Cellulase
- Chitosan
- Immobilized
- Microsphere
- Sponge
ASJC Scopus subject areas
- Biotechnology
- Chemical Engineering(all)
- Renewable Energy, Sustainability and the Environment
- Fuel Technology
- Waste Management and Disposal
- Pollution
- Organic Chemistry
- Inorganic Chemistry