A novel method to prepare chitosan powder and its application in cellulase immobilization

Xuepu Mao; Gangjun Guo; Jinfeng Huang; Zhiyun Du; Zhishu Huang; Lin Ma; Pei Li; Lianquan Gu

doi:10.1002/jctb.1378

A novel method to prepare chitosan powder and its application in cellulase immobilization

Xuepu Mao, Gangjun Guo, Jinfeng Huang, Zhiyun Du, Zhishu Huang, Lin Ma, Pei Li, Lianquan Gu

Research output: Journal article publication › Journal article › Academic research › peer-review

51 Citations (Scopus)

Abstract

In this study, chitosan microspheres and sponges with uniform spherical and porous morphologies were prepared by coiling the stretched chains of chitosan with addition of salt and choosing different kinds of organic solvents as evaporation solvents. Cellulase was immobilized to the support by a covalent method. The enzyme exhibited a considerable affinity to the support, and the protein loading of 145.5 mg g-1support was fairly high. The immobilized cellulase had a higher Kmthan free cellulase and had better stability with respect to pH, thermal stability, reuses and storage stability than free cellulase.

Original language	English
Pages (from-to)	189-195
Number of pages	7
Journal	Journal of Chemical Technology and Biotechnology
Volume	81
Issue number	2
DOIs	https://doi.org/10.1002/jctb.1378
Publication status	Published - 1 Feb 2006

Keywords

Ammonium sulfate
Cellulase
Chitosan
Immobilized
Microsphere
Sponge

ASJC Scopus subject areas

Biotechnology
Chemical Engineering(all)
Renewable Energy, Sustainability and the Environment
Fuel Technology
Waste Management and Disposal
Pollution
Organic Chemistry
Inorganic Chemistry

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10.1002/jctb.1378

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AU - Mao, Xuepu

AU - Guo, Gangjun

AU - Huang, Jinfeng

AU - Du, Zhiyun

AU - Huang, Zhishu

AU - Ma, Lin

AU - Li, Pei

AU - Gu, Lianquan

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AB - In this study, chitosan microspheres and sponges with uniform spherical and porous morphologies were prepared by coiling the stretched chains of chitosan with addition of salt and choosing different kinds of organic solvents as evaporation solvents. Cellulase was immobilized to the support by a covalent method. The enzyme exhibited a considerable affinity to the support, and the protein loading of 145.5 mg g-1support was fairly high. The immobilized cellulase had a higher Kmthan free cellulase and had better stability with respect to pH, thermal stability, reuses and storage stability than free cellulase.

KW - Ammonium sulfate

KW - Cellulase

KW - Chitosan

KW - Immobilized

KW - Microsphere

KW - Sponge

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SN - 0268-2575

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ER -

A novel method to prepare chitosan powder and its application in cellulase immobilization

Abstract

Keywords

ASJC Scopus subject areas

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