A novel fluorescent biosensor for detection of target DNA fragment from the transgene cauliflower mosaic virus 35S promoter

Bin Qiu, Ya shan zhang, Yi bing Lin, Yu Jing Lu, Zhen yu Lin, Kwok Yin Wong, Guo nan Chen

Research output: Journal article publicationJournal articleAcademic researchpeer-review

21 Citations (Scopus)

Abstract

In this paper, we reported a convenient fluorescence method for the detection of genetically modified organisms (GMOs). As it is known that the cauliflower mosaic virus (CaMV) 35S promoter is widely used in most transgenic plants (Schnurr and Guerra, 2000), we thus design a simple method based on the detection of a section target DNA (DNA-T) from the transgene CaMV 35S promoter. In this method, the full-length guanine-rich single-strand sequences were split into fragments (Probe 1 and 2) and each part of the fragment possesses two GGG repeats. In the presence of K+ion and berberine, if a complementary target DNA of the CaMV 35S promoter was introduced to hybridize with Probe 1 and 2, a G-quadruplex-berberine complex was thus formed and generated a strong fluorescence signal. The generation of fluorescence signal indicates the presence of CaMV 35S promoter. This method is able to identify and quantify Genetically Modified Organisms (GMOs), and it shows wide linear ranges from 5.0×10-9to 9.0×10-7mol/L with a detection limit of 2.0×10-9mol/L.
Original languageEnglish
Pages (from-to)168-171
Number of pages4
JournalBiosensors and Bioelectronics
Volume41
Issue number1
DOIs
Publication statusPublished - 15 Mar 2013

Keywords

  • Berberine
  • Cauliflower mosaic virus 35S
  • Fluorescent biosensor
  • G-quadruplex
  • Genetically modified organisms

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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