A novel experimental model of acute hypertriglyceridemia induced by schisandrin B

Si Yuan Pan, Hang Dong, Yifan Han, Wen Yuan Li, Xing Y. Zhao, Kam Ming Ko

Research output: Journal article publicationJournal articleAcademic researchpeer-review

24 Citations (Scopus)

Abstract

Mice were intragastrically treated with single doses (0.05-0.8 g/kg) of schisandrin B (a dibenzocyclooctadiene derivative isolated from the fruit of Schisandra chinensis). Twenty-four hours after schisandrin B administration, the serum triglyceride level was increased by 10-235% in a dose-dependent manner. However, the serum low density lipoprotein cholesterol level was significantly decreased by 28% at a dose of 0.8 g/kg. When given once daily (0.01-0.2 g/kg) for 4 days, schisandrin B also dose-dependently elevated the serum triglyceride level (17-134%). Kinetics parameters estimated by Scott's plot analysis of schisandrin B-induced changes in serum and hepatic triglyceride levels were determined: serum-Emax(maximal effect) = 6 mmol/L (384% increase, P < 0.001); KD(affinity) = 0.59 mmol/kg; pD2(an index of affinity) = 6.62; liver-Emax= 21 μmol/g (68% increase, P < 0.001); KD= 0.37 mmol/kg; pD2= 6.83. The efficacy of schisandrin B in increasing the triglyceride level was 5.6-fold higher in serum than in liver tissue. Fenofibrate (0.2 g/kg) treatment, when in combination with schisandrin B (0.2 g/kg), for 4 days significantly reduced the schisandrin B-induced increase in serum triglyceride level (by 81%, P < 0.001). Hepatic triglyceride level was also decreased (by 100%, P < 0.001) by co-treatment with fenofibrate. Our results suggest that schisandrin B treatment can be used to establish a mouse model of acute hypertrigylceridemia.
Original languageEnglish
Pages (from-to)200-204
Number of pages5
JournalEuropean Journal of Pharmacology
Volume537
Issue number1-3
DOIs
Publication statusPublished - 10 May 2006
Externally publishedYes

Keywords

  • Fenofibrate
  • Hypertriglyceridemia
  • Schisandrin B
  • Total cholesterol
  • Triglyceride

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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