A Newly Discovered Verotoxin Variant, VT2g, Produced by Bovine Verocytotoxigenic Escherichia coli

Hang Mei Polly Leung, J. S.M. Peiris, W. W.S. Ng, R. M. Robins-Browne, K. A. Bettelheim, W. C. Yam

Research output: Journal article publicationJournal articleAcademic researchpeer-review

75 Citations (Scopus)

Abstract

A new verotoxin (VT) variant, designated vt2g, was identified from a bovine strain of verocytotoxigenic Escherichia coli (VTEC) serotype O2:H25. When vt2g was aligned with published sequences of vt2 and vt variants, it exhibited the highest DNA sequence homology with vt2 and vt2c. However, vt2g was not detected by vt2-specific primers and probes, although it was partially neutralized by an antiserum to the VT2A subunit. VT2g was cytotoxic for Vero and HeLa cells and was not activated by mouse intestinal mucus. The vt2g gene was detected in 3 of 409 (0.7%) bovine VTEC strains, including serotypes O2:H25, O2:H45 and Ont:H-.
Original languageEnglish
Pages (from-to)7549-7553
Number of pages5
JournalApplied and Environmental Microbiology
Volume69
Issue number12
DOIs
Publication statusPublished - 1 Dec 2003
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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