A Newly Discovered Verotoxin Variant, VT2g, Produced by Bovine Verocytotoxigenic Escherichia coli

Hang Mei Polly Leung; J. S.M. Peiris; W. W.S. Ng; R. M. Robins-Browne; K. A. Bettelheim; W. C. Yam

doi:10.1128/AEM.69.12.7549-7553.2003

A Newly Discovered Verotoxin Variant, VT2g, Produced by Bovine Verocytotoxigenic Escherichia coli

Hang Mei Polly Leung, J. S.M. Peiris, W. W.S. Ng, R. M. Robins-Browne, K. A. Bettelheim, W. C. Yam

Research output: Journal article publication › Journal article › Academic research › peer-review

85 Citations (Scopus)

Abstract

A new verotoxin (VT) variant, designated vt2g, was identified from a bovine strain of verocytotoxigenic Escherichia coli (VTEC) serotype O2:H25. When vt2g was aligned with published sequences of vt2 and vt variants, it exhibited the highest DNA sequence homology with vt2 and vt2c. However, vt2g was not detected by vt2-specific primers and probes, although it was partially neutralized by an antiserum to the VT2A subunit. VT2g was cytotoxic for Vero and HeLa cells and was not activated by mouse intestinal mucus. The vt2g gene was detected in 3 of 409 (0.7%) bovine VTEC strains, including serotypes O2:H25, O2:H45 and Ont:H-.

Original language	English
Pages (from-to)	7549-7553
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	69
Issue number	12
DOIs	https://doi.org/10.1128/AEM.69.12.7549-7553.2003
Publication status	Published - 1 Dec 2003
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

More information

10.1128/AEM.69.12.7549-7553.2003

Cite this

@article{c53ef96ab0fb4b1dbb4b3be55df1bb53,

title = "A Newly Discovered Verotoxin Variant, VT2g, Produced by Bovine Verocytotoxigenic Escherichia coli",

abstract = "A new verotoxin (VT) variant, designated vt2g, was identified from a bovine strain of verocytotoxigenic Escherichia coli (VTEC) serotype O2:H25. When vt2g was aligned with published sequences of vt2 and vt variants, it exhibited the highest DNA sequence homology with vt2 and vt2c. However, vt2g was not detected by vt2-specific primers and probes, although it was partially neutralized by an antiserum to the VT2A subunit. VT2g was cytotoxic for Vero and HeLa cells and was not activated by mouse intestinal mucus. The vt2g gene was detected in 3 of 409 (0.7%) bovine VTEC strains, including serotypes O2:H25, O2:H45 and Ont:H-.",

author = "Leung, {Hang Mei Polly} and Peiris, {J. S.M.} and Ng, {W. W.S.} and Robins-Browne, {R. M.} and Bettelheim, {K. A.} and Yam, {W. C.}",

year = "2003",

month = dec,

day = "1",

doi = "10.1128/AEM.69.12.7549-7553.2003",

language = "English",

volume = "69",

pages = "7549--7553",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "12",

}

TY - JOUR

T1 - A Newly Discovered Verotoxin Variant, VT2g, Produced by Bovine Verocytotoxigenic Escherichia coli

AU - Leung, Hang Mei Polly

AU - Peiris, J. S.M.

AU - Ng, W. W.S.

AU - Robins-Browne, R. M.

AU - Bettelheim, K. A.

AU - Yam, W. C.

PY - 2003/12/1

Y1 - 2003/12/1

N2 - A new verotoxin (VT) variant, designated vt2g, was identified from a bovine strain of verocytotoxigenic Escherichia coli (VTEC) serotype O2:H25. When vt2g was aligned with published sequences of vt2 and vt variants, it exhibited the highest DNA sequence homology with vt2 and vt2c. However, vt2g was not detected by vt2-specific primers and probes, although it was partially neutralized by an antiserum to the VT2A subunit. VT2g was cytotoxic for Vero and HeLa cells and was not activated by mouse intestinal mucus. The vt2g gene was detected in 3 of 409 (0.7%) bovine VTEC strains, including serotypes O2:H25, O2:H45 and Ont:H-.

AB - A new verotoxin (VT) variant, designated vt2g, was identified from a bovine strain of verocytotoxigenic Escherichia coli (VTEC) serotype O2:H25. When vt2g was aligned with published sequences of vt2 and vt variants, it exhibited the highest DNA sequence homology with vt2 and vt2c. However, vt2g was not detected by vt2-specific primers and probes, although it was partially neutralized by an antiserum to the VT2A subunit. VT2g was cytotoxic for Vero and HeLa cells and was not activated by mouse intestinal mucus. The vt2g gene was detected in 3 of 409 (0.7%) bovine VTEC strains, including serotypes O2:H25, O2:H45 and Ont:H-.

UR - http://www.scopus.com/inward/record.url?scp=2142743315&partnerID=8YFLogxK

U2 - 10.1128/AEM.69.12.7549-7553.2003

DO - 10.1128/AEM.69.12.7549-7553.2003

M3 - Journal article

C2 - 14660413

SN - 0099-2240

VL - 69

SP - 7549

EP - 7553

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 12

ER -

A Newly Discovered Verotoxin Variant, VT2g, Produced by Bovine Verocytotoxigenic Escherichia coli

Abstract

ASJC Scopus subject areas

More information

Other files and links

Fingerprint

Cite this