A new fluorescent biosensor based on inner filter effect and competitive coordination with the europium ion of non-luminescent Eu-MOF nanosheets for the determination of alkaline phosphatase activity in human serum

Shuisheng Hu; Jingyan Liu; Yong Wang; Zhiguang Liang; Bin Hu; Jiahe Xie; Wing Leung Wong; Kwok Yin Wong; Bin Qiu; Weijie Peng

doi:10.1016/j.snb.2023.133379

A new fluorescent biosensor based on inner filter effect and competitive coordination with the europium ion of non-luminescent Eu-MOF nanosheets for the determination of alkaline phosphatase activity in human serum

Shuisheng Hu
, Jingyan Liu
, Yong Wang
, Zhiguang Liang
, Bin Hu
, Jiahe Xie
, Wing Leung Wong
, Kwok Yin Wong
, Bin Qiu
, Weijie Peng

Research output: Journal article publication › Journal article › Academic research › peer-review

41 Citations (Scopus)

Abstract

The abnormal level of alkaline phosphatase (ALP) in human blood stream indicates health problem. A novel fluorescent sensing system based on competitive coordination with europium ions (Eu³⁺) integrated in a non-luminescent lanthanide-metal organic framework nanosheets (NO₂-Eu-MOF NS) was developed for ALP analysis in human serum. The fluorescence signal is turned-on by the antenna ligand (1,4-phenylenediphosphonic acid (PEPP)) that competitively coordinates with Eu³⁺ ions from the nanosheet to produce emissive species (Eu_x(PEPP)_y(NO₂BDC)_z) in-situ. 4-Nitrophenyl phosphate (PNPP) is a substrate of ALP and specifically hydrolyzed into 4-nitrophenol. PNPP exhibits a strong absorption in the UV-Vis region (250–400 nm), which covers the excitation wavelength (280 nm) of Eu_x(PEPP)_y(NO₂BDC)_z. Therefore, the existing of PNPP causes Eu_x(PEPP)_y(NO₂BDC)_z non-emissive and, after hydrolysis with ALP, Eu_x(PEPP)_y(NO₂BDC)_z can be excited and give a detectable fluorescence signal. Based on the fluorescence change, quantitative analysis of ALP activity is thus achievable. This biosensing system was demonstrated in the determination of ALP activity with good linear relationship in the range of 5–1000 U L⁻¹. The limit of detection was 1.1 U L⁻¹ (S/N = 3). The result reveals that the biosensor exhibits excellent sensing performance and anti-interference ability. It could be potentially utilized for clinical routine analysis of ALP activity in human serum.

Original language	English
Article number	133379
Journal	Sensors and Actuators B: Chemical
Volume	380
DOIs	https://doi.org/10.1016/j.snb.2023.133379
Publication status	Published - 1 Apr 2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Alkaline phosphatase
Competitive coordination
Inner filter effect
Nitro-modified lanthanide metal organic framework
Two-dimensional nanosheet

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

More information

10.1016/j.snb.2023.133379

http://hdl.handle.net/10397/97086

Fingerprint

Dive into the research topics of 'A new fluorescent biosensor based on inner filter effect and competitive coordination with the europium ion of non-luminescent Eu-MOF nanosheets for the determination of alkaline phosphatase activity in human serum'. Together they form a unique fingerprint.

Cite this

Hu, S., Liu, J., Wang, Y., Liang, Z., Hu, B., Xie, J., Wong, W. L., Wong, K. Y., Qiu, B., & Peng, W. (2023). A new fluorescent biosensor based on inner filter effect and competitive coordination with the europium ion of non-luminescent Eu-MOF nanosheets for the determination of alkaline phosphatase activity in human serum. Sensors and Actuators B: Chemical, 380, Article 133379. https://doi.org/10.1016/j.snb.2023.133379

@article{a4822cca65534fbcbc01f2866ccea0dc,

title = "A new fluorescent biosensor based on inner filter effect and competitive coordination with the europium ion of non-luminescent Eu-MOF nanosheets for the determination of alkaline phosphatase activity in human serum",

abstract = "The abnormal level of alkaline phosphatase (ALP) in human blood stream indicates health problem. A novel fluorescent sensing system based on competitive coordination with europium ions (Eu3+) integrated in a non-luminescent lanthanide-metal organic framework nanosheets (NO2-Eu-MOF NS) was developed for ALP analysis in human serum. The fluorescence signal is turned-on by the antenna ligand (1,4-phenylenediphosphonic acid (PEPP)) that competitively coordinates with Eu3+ ions from the nanosheet to produce emissive species (Eux(PEPP)y(NO2BDC)z) in-situ. 4-Nitrophenyl phosphate (PNPP) is a substrate of ALP and specifically hydrolyzed into 4-nitrophenol. PNPP exhibits a strong absorption in the UV-Vis region (250–400 nm), which covers the excitation wavelength (280 nm) of Eux(PEPP)y(NO2BDC)z. Therefore, the existing of PNPP causes Eux(PEPP)y(NO2BDC)z non-emissive and, after hydrolysis with ALP, Eux(PEPP)y(NO2BDC)z can be excited and give a detectable fluorescence signal. Based on the fluorescence change, quantitative analysis of ALP activity is thus achievable. This biosensing system was demonstrated in the determination of ALP activity with good linear relationship in the range of 5–1000 U L−1. The limit of detection was 1.1 U L−1 (S/N = 3). The result reveals that the biosensor exhibits excellent sensing performance and anti-interference ability. It could be potentially utilized for clinical routine analysis of ALP activity in human serum.",

keywords = "Alkaline phosphatase, Competitive coordination, Inner filter effect, Nitro-modified lanthanide metal organic framework, Two-dimensional nanosheet",

author = "Shuisheng Hu and Jingyan Liu and Yong Wang and Zhiguang Liang and Bin Hu and Jiahe Xie and Wong, \{Wing Leung\} and Wong, \{Kwok Yin\} and Bin Qiu and Weijie Peng",

note = "Funding Information: The authors gratefully acknowledge the financial support of the Health and Medical Research Fund (HMRF), Hong Kong SAR (Project No. 19200231 ); PolyU Startup Fund (No. P0035712, P0043a754 ); PolyU SZRI Fund (No. P0039278 ). The Jiangxi Provincial Natural Science Foundation (No. 20224BAB213008 ), The Open Project Program of Key Laboratory for Analytical Science of Food Safety and Biology, Ministry of Education (No. FS2201 ). The startup fund (No. QD202134 ) support from the Gannan medical university. Publisher Copyright: {\textcopyright} 2023 Elsevier B.V.",

year = "2023",

month = apr,

day = "1",

doi = "10.1016/j.snb.2023.133379",

language = "English",

volume = "380",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

}

Hu, S, Liu, J, Wang, Y, Liang, Z, Hu, B, Xie, J, Wong, WL , Wong, KY, Qiu, B & Peng, W 2023, 'A new fluorescent biosensor based on inner filter effect and competitive coordination with the europium ion of non-luminescent Eu-MOF nanosheets for the determination of alkaline phosphatase activity in human serum', Sensors and Actuators B: Chemical, vol. 380, 133379. https://doi.org/10.1016/j.snb.2023.133379

A new fluorescent biosensor based on inner filter effect and competitive coordination with the europium ion of non-luminescent Eu-MOF nanosheets for the determination of alkaline phosphatase activity in human serum. / Hu, Shuisheng; Liu, Jingyan; Wang, Yong et al.
In: Sensors and Actuators B: Chemical, Vol. 380, 133379, 01.04.2023.

Research output: Journal article publication › Journal article › Academic research › peer-review

TY - JOUR

T1 - A new fluorescent biosensor based on inner filter effect and competitive coordination with the europium ion of non-luminescent Eu-MOF nanosheets for the determination of alkaline phosphatase activity in human serum

AU - Hu, Shuisheng

AU - Liu, Jingyan

AU - Wang, Yong

AU - Liang, Zhiguang

AU - Hu, Bin

AU - Xie, Jiahe

AU - Wong, Wing Leung

AU - Wong, Kwok Yin

AU - Qiu, Bin

AU - Peng, Weijie

N1 - Funding Information: The authors gratefully acknowledge the financial support of the Health and Medical Research Fund (HMRF), Hong Kong SAR (Project No. 19200231 ); PolyU Startup Fund (No. P0035712, P0043a754 ); PolyU SZRI Fund (No. P0039278 ). The Jiangxi Provincial Natural Science Foundation (No. 20224BAB213008 ), The Open Project Program of Key Laboratory for Analytical Science of Food Safety and Biology, Ministry of Education (No. FS2201 ). The startup fund (No. QD202134 ) support from the Gannan medical university. Publisher Copyright: © 2023 Elsevier B.V.

PY - 2023/4/1

Y1 - 2023/4/1

N2 - The abnormal level of alkaline phosphatase (ALP) in human blood stream indicates health problem. A novel fluorescent sensing system based on competitive coordination with europium ions (Eu3+) integrated in a non-luminescent lanthanide-metal organic framework nanosheets (NO2-Eu-MOF NS) was developed for ALP analysis in human serum. The fluorescence signal is turned-on by the antenna ligand (1,4-phenylenediphosphonic acid (PEPP)) that competitively coordinates with Eu3+ ions from the nanosheet to produce emissive species (Eux(PEPP)y(NO2BDC)z) in-situ. 4-Nitrophenyl phosphate (PNPP) is a substrate of ALP and specifically hydrolyzed into 4-nitrophenol. PNPP exhibits a strong absorption in the UV-Vis region (250–400 nm), which covers the excitation wavelength (280 nm) of Eux(PEPP)y(NO2BDC)z. Therefore, the existing of PNPP causes Eux(PEPP)y(NO2BDC)z non-emissive and, after hydrolysis with ALP, Eux(PEPP)y(NO2BDC)z can be excited and give a detectable fluorescence signal. Based on the fluorescence change, quantitative analysis of ALP activity is thus achievable. This biosensing system was demonstrated in the determination of ALP activity with good linear relationship in the range of 5–1000 U L−1. The limit of detection was 1.1 U L−1 (S/N = 3). The result reveals that the biosensor exhibits excellent sensing performance and anti-interference ability. It could be potentially utilized for clinical routine analysis of ALP activity in human serum.

AB - The abnormal level of alkaline phosphatase (ALP) in human blood stream indicates health problem. A novel fluorescent sensing system based on competitive coordination with europium ions (Eu3+) integrated in a non-luminescent lanthanide-metal organic framework nanosheets (NO2-Eu-MOF NS) was developed for ALP analysis in human serum. The fluorescence signal is turned-on by the antenna ligand (1,4-phenylenediphosphonic acid (PEPP)) that competitively coordinates with Eu3+ ions from the nanosheet to produce emissive species (Eux(PEPP)y(NO2BDC)z) in-situ. 4-Nitrophenyl phosphate (PNPP) is a substrate of ALP and specifically hydrolyzed into 4-nitrophenol. PNPP exhibits a strong absorption in the UV-Vis region (250–400 nm), which covers the excitation wavelength (280 nm) of Eux(PEPP)y(NO2BDC)z. Therefore, the existing of PNPP causes Eux(PEPP)y(NO2BDC)z non-emissive and, after hydrolysis with ALP, Eux(PEPP)y(NO2BDC)z can be excited and give a detectable fluorescence signal. Based on the fluorescence change, quantitative analysis of ALP activity is thus achievable. This biosensing system was demonstrated in the determination of ALP activity with good linear relationship in the range of 5–1000 U L−1. The limit of detection was 1.1 U L−1 (S/N = 3). The result reveals that the biosensor exhibits excellent sensing performance and anti-interference ability. It could be potentially utilized for clinical routine analysis of ALP activity in human serum.

KW - Alkaline phosphatase

KW - Competitive coordination

KW - Inner filter effect

KW - Nitro-modified lanthanide metal organic framework

KW - Two-dimensional nanosheet

UR - https://www.scopus.com/pages/publications/85146566606

U2 - 10.1016/j.snb.2023.133379

DO - 10.1016/j.snb.2023.133379

M3 - Journal article

AN - SCOPUS:85146566606

SN - 0925-4005

VL - 380

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

M1 - 133379

ER -

A new fluorescent biosensor based on inner filter effect and competitive coordination with the europium ion of non-luminescent Eu-MOF nanosheets for the determination of alkaline phosphatase activity in human serum

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

More information

Other files and links

Fingerprint

Cite this