TY - JOUR
T1 - A new fluorescent biosensor based on inner filter effect and competitive coordination with the europium ion of non-luminescent Eu-MOF nanosheets for the determination of alkaline phosphatase activity in human serum
AU - Hu, Shuisheng
AU - Liu, Jingyan
AU - Wang, Yong
AU - Liang, Zhiguang
AU - Hu, Bin
AU - Xie, Jiahe
AU - Wong, Wing Leung
AU - Wong, Kwok Yin
AU - Qiu, Bin
AU - Peng, Weijie
N1 - Funding Information:
The authors gratefully acknowledge the financial support of the Health and Medical Research Fund (HMRF), Hong Kong SAR (Project No. 19200231 ); PolyU Startup Fund (No. P0035712, P0043a754 ); PolyU SZRI Fund (No. P0039278 ). The Jiangxi Provincial Natural Science Foundation (No. 20224BAB213008 ), The Open Project Program of Key Laboratory for Analytical Science of Food Safety and Biology, Ministry of Education (No. FS2201 ). The startup fund (No. QD202134 ) support from the Gannan medical university.
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/4/1
Y1 - 2023/4/1
N2 - The abnormal level of alkaline phosphatase (ALP) in human blood stream indicates health problem. A novel fluorescent sensing system based on competitive coordination with europium ions (Eu3+) integrated in a non-luminescent lanthanide-metal organic framework nanosheets (NO2-Eu-MOF NS) was developed for ALP analysis in human serum. The fluorescence signal is turned-on by the antenna ligand (1,4-phenylenediphosphonic acid (PEPP)) that competitively coordinates with Eu3+ ions from the nanosheet to produce emissive species (Eux(PEPP)y(NO2BDC)z) in-situ. 4-Nitrophenyl phosphate (PNPP) is a substrate of ALP and specifically hydrolyzed into 4-nitrophenol. PNPP exhibits a strong absorption in the UV-Vis region (250–400 nm), which covers the excitation wavelength (280 nm) of Eux(PEPP)y(NO2BDC)z. Therefore, the existing of PNPP causes Eux(PEPP)y(NO2BDC)z non-emissive and, after hydrolysis with ALP, Eux(PEPP)y(NO2BDC)z can be excited and give a detectable fluorescence signal. Based on the fluorescence change, quantitative analysis of ALP activity is thus achievable. This biosensing system was demonstrated in the determination of ALP activity with good linear relationship in the range of 5–1000 U L−1. The limit of detection was 1.1 U L−1 (S/N = 3). The result reveals that the biosensor exhibits excellent sensing performance and anti-interference ability. It could be potentially utilized for clinical routine analysis of ALP activity in human serum.
AB - The abnormal level of alkaline phosphatase (ALP) in human blood stream indicates health problem. A novel fluorescent sensing system based on competitive coordination with europium ions (Eu3+) integrated in a non-luminescent lanthanide-metal organic framework nanosheets (NO2-Eu-MOF NS) was developed for ALP analysis in human serum. The fluorescence signal is turned-on by the antenna ligand (1,4-phenylenediphosphonic acid (PEPP)) that competitively coordinates with Eu3+ ions from the nanosheet to produce emissive species (Eux(PEPP)y(NO2BDC)z) in-situ. 4-Nitrophenyl phosphate (PNPP) is a substrate of ALP and specifically hydrolyzed into 4-nitrophenol. PNPP exhibits a strong absorption in the UV-Vis region (250–400 nm), which covers the excitation wavelength (280 nm) of Eux(PEPP)y(NO2BDC)z. Therefore, the existing of PNPP causes Eux(PEPP)y(NO2BDC)z non-emissive and, after hydrolysis with ALP, Eux(PEPP)y(NO2BDC)z can be excited and give a detectable fluorescence signal. Based on the fluorescence change, quantitative analysis of ALP activity is thus achievable. This biosensing system was demonstrated in the determination of ALP activity with good linear relationship in the range of 5–1000 U L−1. The limit of detection was 1.1 U L−1 (S/N = 3). The result reveals that the biosensor exhibits excellent sensing performance and anti-interference ability. It could be potentially utilized for clinical routine analysis of ALP activity in human serum.
KW - Alkaline phosphatase
KW - Competitive coordination
KW - Inner filter effect
KW - Nitro-modified lanthanide metal organic framework
KW - Two-dimensional nanosheet
UR - http://www.scopus.com/inward/record.url?scp=85146566606&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2023.133379
DO - 10.1016/j.snb.2023.133379
M3 - Journal article
AN - SCOPUS:85146566606
SN - 0925-4005
VL - 380
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
M1 - 133379
ER -