A luminescence-based scanning respirometer for heavy metal toxicity monitoring

Kwok Yin Wong; Min Quan Zhang; Xiang Ming Li; Wai Hung Lo

doi:10.1016/S0956-5663(97)87058-3

A luminescence-based scanning respirometer for heavy metal toxicity monitoring

Kwok Yin Wong, Min Quan Zhang, Xiang Ming Li, Wai Hung Lo

Research output: Journal article publication › Journal article › Academic research › peer-review

38 Citations (Scopus)

Abstract

A respirometer has been developed for microbial toxicity tests. The measurement of oxygen concentration in the respirometer is based on luminescence quenching by molecular oxygen. The oxygen-sensing film consists of a luminescent ruthenium complex adsorbed on amorphous fumed silica and immobilized inside silicone rubber. The luminescence intensity depends on the oxygen concentration in the sample in contact with the sensing film. The inhibition effect of heavy metals on the respiration rate of microorganisms can be evaluated by monitoring the luminescence intensity of the sensing film. The toxicity of Cu2+, Zn2+, Ni2+, Cr3+, Pb2+and Hg2+on activated sludge was tested using the respirometer. The results indicated that the proposed method is a sensitive method for estimating IC50(concentration of a toxicant producing 50% respiration inhibition) in microbial toxicity tests.

Original language	English
Pages (from-to)	125-133
Number of pages	9
Journal	Biosensors and Bioelectronics
Volume	12
Issue number	2
DOIs	https://doi.org/10.1016/S0956-5663(97)87058-3
Publication status	Published - 1 Jan 1997

Keywords

Heavy metals
IC 50
Luminescence quenching
Respirometer
Toxicity test

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/S0956-5663(97)87058-3

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title = "A luminescence-based scanning respirometer for heavy metal toxicity monitoring",

abstract = "A respirometer has been developed for microbial toxicity tests. The measurement of oxygen concentration in the respirometer is based on luminescence quenching by molecular oxygen. The oxygen-sensing film consists of a luminescent ruthenium complex adsorbed on amorphous fumed silica and immobilized inside silicone rubber. The luminescence intensity depends on the oxygen concentration in the sample in contact with the sensing film. The inhibition effect of heavy metals on the respiration rate of microorganisms can be evaluated by monitoring the luminescence intensity of the sensing film. The toxicity of Cu2+, Zn2+, Ni2+, Cr3+, Pb2+and Hg2+on activated sludge was tested using the respirometer. The results indicated that the proposed method is a sensitive method for estimating IC50(concentration of a toxicant producing 50% respiration inhibition) in microbial toxicity tests.",

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AU - Zhang, Min Quan

AU - Li, Xiang Ming

AU - Lo, Wai Hung

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Y1 - 1997/1/1

N2 - A respirometer has been developed for microbial toxicity tests. The measurement of oxygen concentration in the respirometer is based on luminescence quenching by molecular oxygen. The oxygen-sensing film consists of a luminescent ruthenium complex adsorbed on amorphous fumed silica and immobilized inside silicone rubber. The luminescence intensity depends on the oxygen concentration in the sample in contact with the sensing film. The inhibition effect of heavy metals on the respiration rate of microorganisms can be evaluated by monitoring the luminescence intensity of the sensing film. The toxicity of Cu2+, Zn2+, Ni2+, Cr3+, Pb2+and Hg2+on activated sludge was tested using the respirometer. The results indicated that the proposed method is a sensitive method for estimating IC50(concentration of a toxicant producing 50% respiration inhibition) in microbial toxicity tests.

AB - A respirometer has been developed for microbial toxicity tests. The measurement of oxygen concentration in the respirometer is based on luminescence quenching by molecular oxygen. The oxygen-sensing film consists of a luminescent ruthenium complex adsorbed on amorphous fumed silica and immobilized inside silicone rubber. The luminescence intensity depends on the oxygen concentration in the sample in contact with the sensing film. The inhibition effect of heavy metals on the respiration rate of microorganisms can be evaluated by monitoring the luminescence intensity of the sensing film. The toxicity of Cu2+, Zn2+, Ni2+, Cr3+, Pb2+and Hg2+on activated sludge was tested using the respirometer. The results indicated that the proposed method is a sensitive method for estimating IC50(concentration of a toxicant producing 50% respiration inhibition) in microbial toxicity tests.

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KW - IC 50

KW - Luminescence quenching

KW - Respirometer

KW - Toxicity test

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EP - 133

JO - Biosensors and Bioelectronics

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A luminescence-based scanning respirometer for heavy metal toxicity monitoring

Abstract

Keywords

ASJC Scopus subject areas

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