A highly selective quinolizinium-based fluorescent probe for cysteine detection

O. Wa-Yi; Wing Cheung Chan; Caifeng Xu; Jie Ren Deng; Ben Chi Bun Ko; Man Kin Wong

doi:10.1039/d1ra06104f

A highly selective quinolizinium-based fluorescent probe for cysteine detection

O. Wa-Yi, Wing Cheung Chan, Caifeng Xu, Jie Ren Deng, Ben Chi Bun Ko, Man Kin Wong

Research output: Journal article publication › Journal article › Academic research › peer-review

14 Citations (Scopus)

Abstract

A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 μM (S/N = 3). It was successfully applied to cysteine detection in living cells with low cytotoxicity and quantitative analysis of spiked mouse serum samples with moderate to good recovery (96-109%).

Original language	English
Pages (from-to)	33294-33299
Number of pages	6
Journal	RSC Advances
Volume	11
Issue number	53
DOIs	https://doi.org/10.1039/d1ra06104f
Publication status	Published - 11 Oct 2021

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

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10.1039/d1ra06104f

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title = "A highly selective quinolizinium-based fluorescent probe for cysteine detection",

abstract = "A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 μM (S/N = 3). It was successfully applied to cysteine detection in living cells with low cytotoxicity and quantitative analysis of spiked mouse serum samples with moderate to good recovery (96-109%).",

author = "O. Wa-Yi and Chan, {Wing Cheung} and Caifeng Xu and Deng, {Jie Ren} and Ko, {Ben Chi Bun} and Wong, {Man Kin}",

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AU - Wa-Yi, O.

AU - Chan, Wing Cheung

AU - Xu, Caifeng

AU - Deng, Jie Ren

AU - Ko, Ben Chi Bun

AU - Wong, Man Kin

N1 - Funding Information: We gratefully acknowledge the nancial support by the Shenz-hen Science and Technology Innovation Commission (JCYJ20170818104257975), the State Key Laboratory of Chemical Biology and Drug Discovery, and The Hong Kong Polytechnic University (G-ZVQZ). Publisher Copyright: © 2021 The Royal Society of Chemistry.

PY - 2021/10/11

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N2 - A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 μM (S/N = 3). It was successfully applied to cysteine detection in living cells with low cytotoxicity and quantitative analysis of spiked mouse serum samples with moderate to good recovery (96-109%).

AB - A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 μM (S/N = 3). It was successfully applied to cysteine detection in living cells with low cytotoxicity and quantitative analysis of spiked mouse serum samples with moderate to good recovery (96-109%).

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JF - RSC Advances

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ER -

A highly selective quinolizinium-based fluorescent probe for cysteine detection

Abstract

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