A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

Dik Lung Ma, Wing Leung Wong, Wai Hong Chung, Fung Yi Chan, Pui Kin So, Tat Shing Lai, Zhong Yuan Zhou, Yun Chung Leung, Kwok Yin Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

186 Citations (Scopus)

Abstract

No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented). KGaA.
Original languageEnglish
Pages (from-to)3735-3739
Number of pages5
JournalAngewandte Chemie - International Edition
Volume47
Issue number20
DOIs
Publication statusPublished - 5 May 2008

Keywords

  • Electrophoresis
  • Histidine
  • Iridium
  • Luminescent probes
  • Protein staining

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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