A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

Dik Lung Ma; Wing Leung Wong; Wai Hong Chung; Fung Yi Chan; Pui Kin So; Tat Shing Lai; Zhong Yuan Zhou; Yun Chung Leung; Kwok Yin Wong

doi:10.1002/anie.200705319

A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

Dik Lung Ma, Wing Leung Wong, Wai Hong Chung, Fung Yi Chan, Pui Kin So, Tat Shing Lai, Zhong Yuan Zhou, Yun Chung Leung, Kwok Yin Wong

Research output: Journal article publication › Journal article › Academic research › peer-review

225 Citations (Scopus)

Abstract

No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented). KGaA.

Original language	English
Pages (from-to)	3735-3739
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	47
Issue number	20
DOIs	https://doi.org/10.1002/anie.200705319
Publication status	Published - 5 May 2008

Keywords

Electrophoresis
Histidine
Iridium
Luminescent probes
Protein staining

ASJC Scopus subject areas

Catalysis
General Chemistry

More information

10.1002/anie.200705319

Cite this

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title = "A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining",

abstract = "No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented). KGaA.",

keywords = "Electrophoresis, Histidine, Iridium, Luminescent probes, Protein staining",

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A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining. / Ma, Dik Lung; Wong, Wing Leung; Chung, Wai Hong et al.
In: Angewandte Chemie - International Edition, Vol. 47, No. 20, 05.05.2008, p. 3735-3739.

Research output: Journal article publication › Journal article › Academic research › peer-review

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AU - Ma, Dik Lung

AU - Wong, Wing Leung

AU - Chung, Wai Hong

AU - Chan, Fung Yi

AU - So, Pui Kin

AU - Lai, Tat Shing

AU - Zhou, Zhong Yuan

AU - Leung, Yun Chung

AU - Wong, Kwok Yin

PY - 2008/5/5

Y1 - 2008/5/5

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AB - No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented). KGaA.

KW - Electrophoresis

KW - Histidine

KW - Iridium

KW - Luminescent probes

KW - Protein staining

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JO - Angewandte Chemie - International Edition

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A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

Abstract

Keywords

ASJC Scopus subject areas

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