A fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) for the detection of mecA gene sequence of Staphylococcus aureus

Jingyu Shi, Chunyu Chan, Yukting Pang, Weiwei Ye, Feng Tian, Jing Lyu, Yu Zhang, Mo Yang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

200 Citations (Scopus)

Abstract

In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100. nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1. nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET biosensor.
Original languageEnglish
Pages (from-to)595-600
Number of pages6
JournalBiosensors and Bioelectronics
Volume67
DOIs
Publication statusPublished - 5 May 2015

Keywords

  • Fluorescence resonance energy transfer (FRET)
  • Foodborne bacteria
  • Gold nanoparticles (AuNPs)
  • Graphene quantum dots (GQDs)

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this