A flavonol glycoside, isolated from roots of panax notoginseng, reduces amyloid-β-induced neurotoxicity in cultured neurons: Signaling transduction and drug development for Alzheimer's disease

Roy C.Y. Choi, Judy T.T. Zhu, K. Wing Leung, Glanice K.Y. Chu, Heidi Q. Xie, Vicky P. Chen, Ken Y.Z. Zheng, David T.W. Lau, Tina T.X. Dong, Peter C.Y. Chow, Yifan Han, Zheng Tao Wang, Karl W.K. Tsim

Research output: Journal article publicationJournal articleAcademic researchpeer-review

51 Citations (Scopus)

Abstract

A Radix Notoginseng flavonol glycoside (RNFG), quercetin 3-O-β-D-xylopyranosyl-β-D-galactopyranoside, was isolated from roots of Panax notoginseng. Among different biological properties tested, RNFG possessed a strong activity in preventing amyloid-β (Aβ)-induced cell death. In an in vitro assay, RNFG inhibited the aggregation of Aβ in a dose-dependent manner. Moreover, application of RNFG in cultured cortical neurons, or PC12 cells, reduced the Aβ-induced cell death in time- and dose-dependent manners, with the suppression of Aβ-induced DNA fragmentation and caspase-3 activation. In cultured neurons, the pre-treatment of RNFG abolished the increase of Ca2+mobilization triggered by Aβ. The neuroprotective properties of RNFG required a specific sugar attachment within the main chemical backbone because the flavonol backbone by itself did not show any protective effect. In memory impairment experiments using the passive avoidance task, the administration of RNFG reduced brain damage in scopolamine-treated rats. These results therefore reveal a novel function of Radix Notoginseng and its flavonol glycoside that could be very useful in developing food supplements for the prevention, or potential treatment, of Alzheimer's disease.
Original languageEnglish
Pages (from-to)795-811
Number of pages17
JournalJournal of Alzheimer's Disease
Volume19
Issue number3
DOIs
Publication statusPublished - 1 Jan 2010

Keywords

  • Alzheimer's disease
  • Chinese medicine
  • Flavonol glycoside
  • Neuroprotection
  • Panax notoginseng

ASJC Scopus subject areas

  • Clinical Psychology
  • Geriatrics and Gerontology
  • Psychiatry and Mental health

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