@article{80854db8cf3b4122b8139986feaa2494,
title = "A Cytoplasm-Specific Fluorescent Ligand for Selective Imaging of RNA G-Quadruplexes in Live Cancer Cells",
abstract = "The development of site-specific, target-selective and biocompatible small molecule ligands as a fluorescent tool for real-time study of cellular functions of RNA G-quadruplexes (G4s), which are associated with human cancers, is of significance in cancer biology. We report a fluorescent ligand that is a cytoplasm-specific and RNA G4-selective fluorescent biosensor in live HeLa cells. The in vitro results show that the ligand is highly selective targeting RNA G4s including VEGF, NRAS, BCL2 and TERRA. These G4s are recognized as human cancer hallmarks. Moreover, intracellular competition studies with BRACO19 and PDS, and the colocalization study with G4-specific antibody (BG4) in HeLa cells may support that the ligand selectively binds to G4s in cellulo. Furthermore, the ligand was demonstrated for the first time in the visualization and monitoring of dynamic resolving process of RNA G4s by the overexpressed RFP-tagged DHX36 helicase in live HeLa cells.",
keywords = "DHX36, fluorescent G4-ligand, G4-structure resolving, live cell imaging, RNA G-quadruplex",
author = "Zheng, {Bo Xin} and Wei Long and She, {Meng Ting} and Yakun Wang and Dong Zhao and Jie Yu and {Siu-Lun Leung}, Alan and {Hin Chan}, Ka and Jinqiang Hou and Lu, {Yu Jing} and Wong, {Wing Leung}",
note = "Funding Information: The work is fully supported by the grant received from the Research Grants Council of the Hong Kong Special Administrative Region, China (RGC Project No. 15300522). W.L acknowledges the award of a postdoctoral fellowship administered by the Research Committee of The Hong Kong Polytechnic University. The University Research Facilities on Life Sciences and Chemical and Environmental Analysis of The Hong Kong Polytechnic University are also acknowledged. Funding Information: The work is fully supported by the grant received from the Research Grants Council of the Hong Kong Special Administrative Region, China (RGC Project No. 15300522). W.L acknowledges the award of a postdoctoral fellowship administered by the Research Committee of The Hong Kong Polytechnic University. The University Research Facilities on Life Sciences and Chemical and Environmental Analysis of The Hong Kong Polytechnic University are also acknowledged. Publisher Copyright: {\textcopyright} 2023 Wiley-VCH GmbH.",
year = "2023",
month = jun,
day = "19",
doi = "10.1002/chem.202300705",
language = "English",
volume = "29",
journal = "Chemistry - A European Journal",
issn = "0947-6539",
publisher = "Wiley-VCH Verlag",
number = "34",
}