A cyanine-modified upconversion nanoprobe for NIR-excited imaging of endogenous hydrogen peroxide signaling invivo

Yi Zhou; Wenbo Pei; Xiao Zhang; Wangqiao Chen; Jiansheng Wu; Cheng Yao; Ling Huang; Hua Zhang; Wei Huang; Joachim Say Chye Loo; Qichun Zhang

doi:10.1016/j.biomaterials.2015.03.003

A cyanine-modified upconversion nanoprobe for NIR-excited imaging of endogenous hydrogen peroxide signaling invivo

Yi Zhou, Wenbo Pei, Xiao Zhang, Wangqiao Chen, Jiansheng Wu, Cheng Yao, Ling Huang, Hua Zhang, Wei Huang, Joachim Say Chye Loo, Qichun Zhang

Research output: Journal article publication › Journal article › Academic research › peer-review

78 Citations (Scopus)

Abstract

Endogenous hydrogen peroxide is an important parameter associated with cellular signal transduction and homeostasis. However, abnormal H₂O₂ regulation in live systems has been implicated in many pathological conditions. Monitoring this signal in live systems is essential but challenging because current H₂O₂ probes are impractical for efficient bio-imaging due to UV/visible light as the excitation source. We herein present a novel design based on an organic fluorophore-attached lanthanide-doped upconversion nanoprobe (CYD1-UCNPs) for selective UCL detection of H₂O₂. This nanoprobe represents the next-generation imaging tool that features a robust UCL "turn-on" response to H₂O₂ with NIR-excited ratiometric signals and has potential applications in ratiometric UCL imaging of endogenous H₂O₂ generating in living cells and whole-body animals.

Original language	English
Pages (from-to)	34-43
Number of pages	10
Journal	Biomaterials
Volume	54
DOIs	https://doi.org/10.1016/j.biomaterials.2015.03.003
Publication status	Published - 1 Jun 2015
Externally published	Yes

Keywords

Hydrogen peroxide
Immune response
NIR-excited imaging
Ratiometric
Upconversion luminescence

ASJC Scopus subject areas

Biophysics
Bioengineering
Ceramics and Composites
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2015.03.003

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title = "A cyanine-modified upconversion nanoprobe for NIR-excited imaging of endogenous hydrogen peroxide signaling invivo",

abstract = "Endogenous hydrogen peroxide is an important parameter associated with cellular signal transduction and homeostasis. However, abnormal H2O2 regulation in live systems has been implicated in many pathological conditions. Monitoring this signal in live systems is essential but challenging because current H2O2 probes are impractical for efficient bio-imaging due to UV/visible light as the excitation source. We herein present a novel design based on an organic fluorophore-attached lanthanide-doped upconversion nanoprobe (CYD1-UCNPs) for selective UCL detection of H2O2. This nanoprobe represents the next-generation imaging tool that features a robust UCL {"}turn-on{"} response to H2O2 with NIR-excited ratiometric signals and has potential applications in ratiometric UCL imaging of endogenous H2O2 generating in living cells and whole-body animals.",

keywords = "Hydrogen peroxide, Immune response, NIR-excited imaging, Ratiometric, Upconversion luminescence",

author = "Yi Zhou and Wenbo Pei and Xiao Zhang and Wangqiao Chen and Jiansheng Wu and Cheng Yao and Ling Huang and Hua Zhang and Wei Huang and {Chye Loo}, {Joachim Say} and Qichun Zhang",

note = "Funding Information: Q.Z. acknowledges financial support from AcRF Tier 1 ( RG 16/12 and RG 133/14 ) and Tier 2 ( ARC 20/12 and ARC 2/13 ) from MOE, and the CREATE program (Nanomaterials for Energy and Water Management) from NRF, Singapore . J. Loo acknowledges the financial support from the Centre of Artificial Photosynthesis (CAPS) , and the Singapore Centre on Environmental Life Sciences Engineering (SCELSE) , NTU . Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",

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doi = "10.1016/j.biomaterials.2015.03.003",

language = "English",

volume = "54",

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AU - Zhou, Yi

AU - Pei, Wenbo

AU - Zhang, Xiao

AU - Chen, Wangqiao

AU - Wu, Jiansheng

AU - Yao, Cheng

AU - Huang, Ling

AU - Zhang, Hua

AU - Huang, Wei

AU - Chye Loo, Joachim Say

AU - Zhang, Qichun

N1 - Funding Information: Q.Z. acknowledges financial support from AcRF Tier 1 ( RG 16/12 and RG 133/14 ) and Tier 2 ( ARC 20/12 and ARC 2/13 ) from MOE, and the CREATE program (Nanomaterials for Energy and Water Management) from NRF, Singapore . J. Loo acknowledges the financial support from the Centre of Artificial Photosynthesis (CAPS) , and the Singapore Centre on Environmental Life Sciences Engineering (SCELSE) , NTU . Publisher Copyright: © 2015 Elsevier Ltd.

PY - 2015/6/1

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N2 - Endogenous hydrogen peroxide is an important parameter associated with cellular signal transduction and homeostasis. However, abnormal H2O2 regulation in live systems has been implicated in many pathological conditions. Monitoring this signal in live systems is essential but challenging because current H2O2 probes are impractical for efficient bio-imaging due to UV/visible light as the excitation source. We herein present a novel design based on an organic fluorophore-attached lanthanide-doped upconversion nanoprobe (CYD1-UCNPs) for selective UCL detection of H2O2. This nanoprobe represents the next-generation imaging tool that features a robust UCL "turn-on" response to H2O2 with NIR-excited ratiometric signals and has potential applications in ratiometric UCL imaging of endogenous H2O2 generating in living cells and whole-body animals.

AB - Endogenous hydrogen peroxide is an important parameter associated with cellular signal transduction and homeostasis. However, abnormal H2O2 regulation in live systems has been implicated in many pathological conditions. Monitoring this signal in live systems is essential but challenging because current H2O2 probes are impractical for efficient bio-imaging due to UV/visible light as the excitation source. We herein present a novel design based on an organic fluorophore-attached lanthanide-doped upconversion nanoprobe (CYD1-UCNPs) for selective UCL detection of H2O2. This nanoprobe represents the next-generation imaging tool that features a robust UCL "turn-on" response to H2O2 with NIR-excited ratiometric signals and has potential applications in ratiometric UCL imaging of endogenous H2O2 generating in living cells and whole-body animals.

KW - Hydrogen peroxide

KW - Immune response

KW - NIR-excited imaging

KW - Ratiometric

KW - Upconversion luminescence

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A cyanine-modified upconversion nanoprobe for NIR-excited imaging of endogenous hydrogen peroxide signaling invivo

Abstract

Keywords

ASJC Scopus subject areas

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