TY - JOUR
T1 - A comparative investigation of the interaction and pharmacokinetics of hemoglobin with berberine and its oxymetabolite
AU - Chen, Han-Bin
AU - Luo, Chao-Dan
AU - Ai, Gao-Xiang
AU - Wang, Yong-Fu
AU - Li, Cai-Lan
AU - Tan, Li-Hua
AU - Lee, Simon Ming-Yuen
AU - Cheung, Alex Kwok-Kuen
AU - Su, Zi-Ren
AU - Wu, Xiao-Li
AU - Xie, Jian-Hui
AU - Zeng, Hui-Fang
N1 - Funding Information:
This work was supported by grants from National Natural Science Foundation of China (Nos. 82074082 & 82003771 ), National Natural Science Foundation of Guangdong, China ( 2019A1515010819 ), Provincial-level Major Scientific Research Projects in Ordinary Universities of Guangdong Province, China ( 2017KZDXM017 ), Key Program for Subject Research of Guangzhou University of Chinese Medicine ( XK2018016 & XK2019002 ), Characteristic Cultivation Program for Subject Research of Guangzhou University of Chinese Medicine ( XKP2019007 ), Special Project on the Integration of Industry, Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine ( 2017B030314096 ), Young Innovative Talents Program, Educational Commission of Guangdong Province, China ( 2019KQNCX024 ), and Guizhou Science and Technology Foundation of China (No. QKHPTRC[2018]5772-021 ).
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/5/30
Y1 - 2021/5/30
N2 - Berberine (BBR), isolated from Coptis chinensis, is one type of isoquinoline alkaloids. BBR exerts numerous of bioactivities but the plasma concentration is really low. In our previous study, a new oxymetabolite (OBB) has been discovered and showed superior anti-inflammatory effect comparing with BBR. The aim of this study is to investigate the interaction, metabolite and pharmacokinetics of BBR with hemoglobin. Sprague-Dawley rats were used to carry out the interaction, metabolite and pharmacokinetics of BBR and OBB in vivo. Fluorescence spectra were used to analyse the interaction in vitro. Results showed that OBB could be generated after intravenous injection or incubating with BBR in vitro and in vivo; Both BBR and OBB exerted much stronger binding interaction with hemoglobin than plasma and affect the conformation of bovine hemoglobin and change the fluorescence spectral properties; BBR and OBB were mainly presented and transported in the proteins-bound form. These results provide a new insight to understand the dynamic equilibrium of BBR and OBB within body from the perspective of new metabolic pathways.
AB - Berberine (BBR), isolated from Coptis chinensis, is one type of isoquinoline alkaloids. BBR exerts numerous of bioactivities but the plasma concentration is really low. In our previous study, a new oxymetabolite (OBB) has been discovered and showed superior anti-inflammatory effect comparing with BBR. The aim of this study is to investigate the interaction, metabolite and pharmacokinetics of BBR with hemoglobin. Sprague-Dawley rats were used to carry out the interaction, metabolite and pharmacokinetics of BBR and OBB in vivo. Fluorescence spectra were used to analyse the interaction in vitro. Results showed that OBB could be generated after intravenous injection or incubating with BBR in vitro and in vivo; Both BBR and OBB exerted much stronger binding interaction with hemoglobin than plasma and affect the conformation of bovine hemoglobin and change the fluorescence spectral properties; BBR and OBB were mainly presented and transported in the proteins-bound form. These results provide a new insight to understand the dynamic equilibrium of BBR and OBB within body from the perspective of new metabolic pathways.
KW - Berberine
KW - Hemoglobin
KW - Interaction
KW - Metabolite
KW - Pharmacokinetics
UR - http://www.scopus.com/inward/record.url?scp=85103121119&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2021.114032
DO - 10.1016/j.jpba.2021.114032
M3 - Journal article
SN - 0731-7085
VL - 199
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
M1 - 114032
ER -