A bioorthogonal time-resolved luminogenic probe for metabolic labelling and imaging of glycans

Judun Zheng, Qiuqiang Zhan, Lijun Jiang, Da Xing, Tao Zhang, Ka Leung Wong (Corresponding Author)

Research output: Journal article publicationJournal articleAcademic researchpeer-review

8 Citations (Scopus)


The use of bioorthogonal fluorogenic probes is superior to labelling and imaging of biomolecules in live cells and organisms, although overcoming the limitation of autofluorescence is still a challenge for current probes to achieve high illumination resolution of the target of interest. We herein demonstrate a functionalized terbium complex Tb-1 that is stable and biocompatible to enable bioorthogonal ligation with engineered cell-surface glycans for providing responsive luminescence. A luminescence resonance energy transfer (LRET) quencher with bioorthogonal properties is strategically incorporated into a tripodal terbium complex with low toxicity, which can undergo a click-cycloaddition reaction with a cyclooctene to completely change the electronic structure of the quencher, resulting in a much less efficient LRET but a 5-fold enhancement in the long-lived terbium emission intensity. This work therefore establishes a time-resolved platform that enables labelling and imaging of the biomolecules of interest. This journal is

Original languageEnglish
Pages (from-to)4062-4069
Number of pages8
JournalInorganic Chemistry Frontiers
Issue number21
Publication statusPublished - 7 Nov 2020
Externally publishedYes

ASJC Scopus subject areas

  • Inorganic Chemistry


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