A 3D cancer-biofilm microfluidic model for disease modelling and drug screening

Yanlin Deng; Song Lin Chua; Bee Luan Khoo

A 3D cancer-biofilm microfluidic model for disease modelling and drug screening

Yanlin Deng
, Song Lin Chua
, Bee Luan Khoo

The Hong Kong Polytechnic University

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

Abstract

We developed an in vitro Platform to investigate how intratumoral bacteria (IB) and extratumoral bacteria (EB) (PIEB) affect tumor progression. Pathogen-cancer cell co-culture models were established to mimic the infecting and colonization states of bacteria in the cancer microenvironment. Based on this microwell-based microfluidic chip, various in situ downstream assays of tumor cell clusters can be applied, and the cancer-protecting effects of extracellular biofilms formed by EB were proved to have protective effects on cancer cells. We further proposed a combinational triple-drug therapy, which could destroy the biofilm structure and kill cancer cells simultaneously to achieve an effective treatment effect.

Original language	English
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	79-80
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - Oct 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 10 Oct 2021 → 14 Oct 2021

Publication series

Name	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 14/10/21

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

biofilms
combinational cancer therapy
Microfluidics
tumor models

ASJC Scopus subject areas

Bioengineering
Chemical Engineering (miscellaneous)

Cite this

Deng, Y., Chua, S. L., & Khoo, B. L. (2021). A 3D cancer-biofilm microfluidic model for disease modelling and drug screening. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 79-80). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Deng, Yanlin ; Chua, Song Lin ; Khoo, Bee Luan. / A 3D cancer-biofilm microfluidic model for disease modelling and drug screening. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 79-80 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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title = "A 3D cancer-biofilm microfluidic model for disease modelling and drug screening",

abstract = "We developed an in vitro Platform to investigate how intratumoral bacteria (IB) and extratumoral bacteria (EB) (PIEB) affect tumor progression. Pathogen-cancer cell co-culture models were established to mimic the infecting and colonization states of bacteria in the cancer microenvironment. Based on this microwell-based microfluidic chip, various in situ downstream assays of tumor cell clusters can be applied, and the cancer-protecting effects of extracellular biofilms formed by EB were proved to have protective effects on cancer cells. We further proposed a combinational triple-drug therapy, which could destroy the biofilm structure and kill cancer cells simultaneously to achieve an effective treatment effect.",

keywords = "biofilms, combinational cancer therapy, Microfluidics, tumor models",

author = "Yanlin Deng and Chua, \{Song Lin\} and Khoo, \{Bee Luan\}",

note = "Funding Information: This study was supported by the City University of Hong Kong, which is funded by the Research Grants Council (RGC), the Hong Kong Polytechnic University (BE2B), Environmental and Conservation Fund (ECF 49/2019), and State Key Laboratory for Chemical Biology and Drug Discovery. Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

month = oct,

language = "English",

series = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

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Deng, Y, Chua, SL & Khoo, BL 2021, A 3D cancer-biofilm microfluidic model for disease modelling and drug screening. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 79-80, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 10/10/21.

A 3D cancer-biofilm microfluidic model for disease modelling and drug screening. / Deng, Yanlin; Chua, Song Lin; Khoo, Bee Luan.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 79-80 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

TY - GEN

T1 - A 3D cancer-biofilm microfluidic model for disease modelling and drug screening

AU - Deng, Yanlin

AU - Chua, Song Lin

AU - Khoo, Bee Luan

N1 - Funding Information: This study was supported by the City University of Hong Kong, which is funded by the Research Grants Council (RGC), the Hong Kong Polytechnic University (BE2B), Environmental and Conservation Fund (ECF 49/2019), and State Key Laboratory for Chemical Biology and Drug Discovery. Publisher Copyright: © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

PY - 2021/10

Y1 - 2021/10

N2 - We developed an in vitro Platform to investigate how intratumoral bacteria (IB) and extratumoral bacteria (EB) (PIEB) affect tumor progression. Pathogen-cancer cell co-culture models were established to mimic the infecting and colonization states of bacteria in the cancer microenvironment. Based on this microwell-based microfluidic chip, various in situ downstream assays of tumor cell clusters can be applied, and the cancer-protecting effects of extracellular biofilms formed by EB were proved to have protective effects on cancer cells. We further proposed a combinational triple-drug therapy, which could destroy the biofilm structure and kill cancer cells simultaneously to achieve an effective treatment effect.

AB - We developed an in vitro Platform to investigate how intratumoral bacteria (IB) and extratumoral bacteria (EB) (PIEB) affect tumor progression. Pathogen-cancer cell co-culture models were established to mimic the infecting and colonization states of bacteria in the cancer microenvironment. Based on this microwell-based microfluidic chip, various in situ downstream assays of tumor cell clusters can be applied, and the cancer-protecting effects of extracellular biofilms formed by EB were proved to have protective effects on cancer cells. We further proposed a combinational triple-drug therapy, which could destroy the biofilm structure and kill cancer cells simultaneously to achieve an effective treatment effect.

KW - biofilms

KW - combinational cancer therapy

KW - Microfluidics

KW - tumor models

UR - https://www.scopus.com/pages/publications/85136933192

M3 - Conference article published in proceeding or book

AN - SCOPUS:85136933192

T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 79

EP - 80

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

Deng Y, Chua SL, Khoo BL. A 3D cancer-biofilm microfluidic model for disease modelling and drug screening. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2021. p. 79-80. (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

A 3D cancer-biofilm microfluidic model for disease modelling and drug screening

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

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Cite this