3D High-Frequency Ultrasound Imaging of Cartilage-Bone Interface Compared with Micro-CT

Chunyi Wen, Yanping Huang, Guangquan Zhou, Yongping Zheng, Chun Hoi Yan, Choi Han Chan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

4 Citations (Scopus)


Cartilage-bone interface (CBI) is a complex structure which bears important information in pathophysiology of osteoarthritis
(OA). While high-frequency ultrasound (US) has been widely used for the investigation of articular cartilage, 3D imaging of CBI
using US is less commonly reported in this field. Here, we adopted a 3D high-frequency ultrasound imaging approach
specifically for the investigation of CBI in human knee samples. Fifteen osteochondral disks from the tibial plateau of seven OA
patients were prepared in vitro and scanned using both high-frequency US and micro-CT imaging. The 3D morphology of the
tidemark was reconstructed and compared using an image registration approach between the two imaging modalities. Results
showed that the 3D tidemark could be well registered between the two imaging methods with a mean surface discrepancy of
33:2±9:9 μm. Quantitative surface waviness/roughness parameter analysis showed significant correlations between the two
imaging modalities. An intensity projected en face imaging was proposed to probe characteristic details of the CBI such as its
perforations. This study provided evidence for the 3D high-frequency ultrasound as a nonionizing radiation imaging tool
potentially useful to evaluate the change of CBI in basic research of join diseases including OA.
Original languageEnglish
Article number 6906148
Number of pages10
JournalBioMed Research International
Publication statusPublished - 1 Jun 2020

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • General Immunology and Microbiology


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